Intense effects of food restriction and overfed regulates glycogen content modulate biochemical and fertilization behaviour in zebrafish (Danio rerio).

Shanmugasundaram Tamilarasan; Indhirani K; Prithika K.; Premkumar R.; Venkatesh D.r; Jose Vinoth Raja; Jayanthi Priya K; King Immanuel

doi:10.5455/JBRR.20251020040312

Abstract
Background:Introduction: Zebrafish have become a valuable model organism in biomedical research precisely because of their many advantages, including their genetic similarity to humans and the transparency of their embryos. The effects of fasting and overfeeding on zebrafish reproductive performance and fertilization ratio can provide valuable insights into the impacts on their offspring.
Methods: The experimental setup you outlined aims to examine the effects on adult zebrafish from two different AB strains and wild-type lines of various feeding regimes, including control, 12-hour intermittent fasting, and overfeeding. Data were collected regularly throughout the 35-day experimental period to monitor changes in the subjects' physical characteristics, biochemical indicators, histology, and reproductive efficiency. Fish that were subjected to fasting exhibited accelerated growth compared to those that were overfed.
Result: This observation suggests that intermittent fasting stimulates growth and reproductive performance in zebrafish by enhancing metabolic efficiency and fertilization performance.
Conclusion: The investigation data indicate that, compared to the overfed group, intermittent fasting may have positive effects on zebrafish reproductive biology generally. It is a's crucial to remember that while fasting may improve reproductive function, it's far from a straight comparison between human obesity and zebrafish reproductive activity. It is necessary to acknowledge that human obesity can negatively influence fertility.

Key words: Fasting, Overfed, Embryo, Fertilization, Zebrafish